Chelsea right back Victor Moses has been dropped from the Super Eagles’ 2019 Africa Cup of Nations qualifier against South Africa next month.
Speculation has been rife in recent weeks over the whether Moses, 26, would be available for the June 10 match in the southern city of Uyo.
Another surprise in Gernot Rohr’s 23-man squad is a first call-up for Maroof Youssef, who plays for Egyptian side Zamalek.
The 24-year-old, who can operate as a defensive midfielder or forward but is listed in the squad as a defender, helped the Cairo club win a league and cup double last season.
They also reached the last four of the 2016 CAF Champions League.
Akwa United star forward Alhassan Ibrahim and goalkeeper Ikechukwu Ezenwa, from FC Ifeanyi Ubah, are the only two players called up from the domestic league.
Goalkeepers: Daniel Akpeyi (Chippa United/RSA), Ikechukwu Ezenwa (FC Ifeanyi Ubah), Dele Alampasu (FC Cesarense/POR)
Defenders: Elderson Echiejile (AS Monaco/FRA), Kenneth Omeruo (Alanyaspor/TUR), William Troost-Ekong (KAA Gent/BEL), Tyronne Ebuehi, (ADO Den Haag/NED), Shehu Abdullahi (Anorthosis Famagusta/CYP), Maroof Youssef (Zamalek/EGY), Chidozie Awaziem (FC Porto/POR)
Midfielders: Wilfred Ndidi (Leicester City/ENG), John Ogu (Hapoel Be’er Sheva/ISR), Ogenyi Onazi (Trabzonspor/TUR), Mikel Agu (FC Porto/POR), Oghenekaro Etebo (Feirense/POR), Alhassan Ibrahim (Akwa United)
Forwards: Henry Onyekuru (KAS Eupen/BEL), Victor Osimhen (Wolfsburg/GER), Kelechi Iheanacho (Manchester City/ENG), Ahmed Musa (Leicester City/ENG), Olanrewaju Kayode (Austria Wien/AUS), Alex Iwobi (Arsenal/ENG), Moses Simon (KAA Gent/BEL)